Hotspot

ndhF

SSC · 113433-115682 · 2250 bp

Back to Cissus

Pi 0.0231
MarkerSeek score 59.80
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2250
variable_sites
111
pi
0.0231
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACTTCCGAAACGAAGGGAAC CGGTGAATCGGAAACAAGTA 50.0 45.0 58.1 55.8 2474-2530 bp 0.4 38.7
ndhF_p2 GAACTTCCGAAACGAAGGGA CGGTGAATCGGAAACAAGTA 50.0 45.0 57.8 55.8 2476-2532 bp 0.4 38.4
ndhF_p3 AGAACTTCCGAAACGAAGGG CGGTGAATCGGAAACAAGTA 50.0 45.0 57.8 55.8 2477-2533 bp 0.4 38.4
ndhF_p4 TAGGCAGAATACCCTCACCC CGGTGAATCGGAAACAAGTA 55.0 45.0 58.2 55.8 2578-2634 bp 0.4 38.1
ndhF_p5 GGAACAAGAGAGATCCACCG CGGTGAATCGGAAACAAGTA 55.0 45.0 57.7 55.8 2448-2504 bp 0.4 37.3