Hotspot

ccsA-ndhD

SSC · 119489-119648 · 160 bp

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Pi 0.07
MarkerSeek score 78.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
160
variable_sites
21
pi
0.07
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TTCTGCAATTGTGGCGTCTA TGGGCGGAATAGCTATCTCA 45.0 50.0 57.8 58.0 697-704 bp 1.0 84.9
ccsA-ndhD_p2 TACAATAACCGGGCCAAGTG TGGGCGGAATAGCTATCTCA 50.0 50.0 57.9 58.0 1252-1259 bp 1.0 83.5
ccsA-ndhD_p3 GTACAATAACCGGGCCAAGT TGGGCGGAATAGCTATCTCA 50.0 50.0 57.9 58.0 1253-1260 bp 1.0 83.5
ccsA-ndhD_p4 GCTAATGAGGCATGGGGATC TGGGCGGAATAGCTATCTCA 55.0 50.0 58.2 58.0 819-826 bp 0.8 75.4
ccsA-ndhD_p5 CTAATGAGGCATGGGGATCG TGGGCGGAATAGCTATCTCA 55.0 50.0 57.9 58.0 818-825 bp 0.6 66.5