Hotspot

psbT-psbN

LSC · 77531-77593 · 63 bp

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Pi 0.119
MarkerSeek score 74.80
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
63
variable_sites
13
pi
0.119
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 TTCTTCGGACACATTTGGCA TCCCCTACGGTAGTTGGTTT 45.0 50.0 58.0 58.0 794-799 bp 1.0 84.0
psbT-psbN_p2 TCCGGATTTGGATGCTCAAG TCCCCTACGGTAGTTGGTTT 50.0 50.0 58.0 58.0 732-737 bp 1.0 84.0
psbT-psbN_p3 CGATCCCGCTACTGTGAAAA TCCCCTACGGTAGTTGGTTT 50.0 50.0 58.0 58.0 945-950 bp 1.0 83.8
psbT-psbN_p4 CGATCCCGCTACTGTGAAAA AGCTAGGACTTCGTGGTTCT 50.0 50.0 58.0 58.1 1132-1137 bp 0.8 74.5
psbT-psbN_p5 TTCTTCGGACACATTTGGCA AGCTAGGACTTCGTGGTTCT 45.0 50.0 58.0 58.1 981-986 bp 0.8 74.5