Hotspot

petA-psbJ

LSC · 65863-66963 · 1101 bp

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Pi 0.0954
MarkerSeek score 88.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1101
variable_sites
186
pi
0.0954
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
88.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCTTCTGTTATTTTGGCACA ATTCCGGATTGGGTTCATCC 42.9 50.0 57.3 57.6 1179-1212 bp 1.0 75.8
petA-psbJ_p2 TGGCTTCTGTTATTTTGGCAC ATTCCGGATTGGGTTCATCC 42.9 50.0 57.3 57.6 1180-1213 bp 1.0 75.8
petA-psbJ_p3 TGAGAAGGTTCAATTGTCCGA ATTCCGGATTGGGTTCATCC 42.9 50.0 57.2 57.6 1128-1161 bp 1.0 75.0
petA-psbJ_p4 TTGGCTTCTGTTATTTTGGCAC ATTCCGGATTGGGTTCATCC 40.9 50.0 58.0 57.6 1181-1214 bp 1.0 73.0
petA-psbJ_p5 TGAGAAGGTTCAATTGTCCGAA ATTCCGGATTGGGTTCATCC 40.9 50.0 57.9 57.6 1128-1161 bp 1.0 72.4