Hotspot

trnY-GUA-trnE-UUC

LSC · 32397-32455 · 59 bp

Back to Cissus

Pi 0.0407
MarkerSeek score 75.40
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
59
variable_sites
6
pi
0.0407
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 TATTGGATCGCTTGTGTCGG AAGTATGCCCCCATCGTCTA 50.0 50.0 58.1 57.9 770-776 bp 0.8 75.7
trnY-GUA-trnE-UUC_p2 TATTGGATCGCTTGTGTCGG TGATCCCTCTTCCCCGATTT 50.0 50.0 58.1 58.1 1243-1270 bp 0.6 66.7
trnY-GUA-trnE-UUC_p3 TATTGGATCGCTTGTGTCGG TTGATCCCTCTTCCCCGATT 50.0 50.0 58.1 58.1 1244-1271 bp 0.6 66.7
trnY-GUA-trnE-UUC_p4 TATTGGATCGCTTGTGTCGG ATTGATCCCTCTTCCCCGAT 50.0 50.0 58.1 57.9 1245-1272 bp 0.6 66.5
trnY-GUA-trnE-UUC_p5 TATTGGATCGCTTGTGTCGG TCCCCTCCCCAATTCTTAGG 50.0 55.0 58.1 58.1 1286 bp 0.2 46.7