Hotspot

trnR-UCU-atpA

LSC · 10562-10785 · 224 bp

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Pi 0.0646
MarkerSeek score 78.10
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
224
variable_sites
32
pi
0.0646
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AGCCTTCCAAGCTAACGATG GGAACGCTTCCTACTTCAGG 50.0 55.0 58.0 58.0 541-622 bp 1.0 90.4
trnR-UCU-atpA_p2 TCGGAATGAAAAGCGTCCAT GGAACGCTTCCTACTTCAGG 45.0 55.0 57.9 58.0 339-417 bp 1.0 90.3
trnR-UCU-atpA_p3 CCTAGCCTTCCAAGCTAACG GGAACGCTTCCTACTTCAGG 55.0 55.0 58.1 58.0 544-625 bp 1.0 90.2
trnR-UCU-atpA_p4 ATCGGAATGAAAAGCGTCCA GGAACGCTTCCTACTTCAGG 45.0 55.0 57.9 58.0 340-418 bp 1.0 90.2
trnR-UCU-atpA_p5 AGCCTTCCAAGCTAACGATG CGAAGCTTATCCGGGAGATG 50.0 55.0 58.0 57.9 1183-1264 bp 1.0 85.9