Hotspot

trnK-UUU-rps16

LSC · 4417-5105 · 689 bp

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Pi 0.0571
MarkerSeek score 82.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
689
variable_sites
80
pi
0.0571
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CAGGATCAGTCGCGGTTTTA TATCCCAATGGGCCGTCTAT 50.0 50.0 58.0 58.0 1043-1118 bp 1.0 88.0
trnK-UUU-rps16_p2 AGGATCAGTCGCGGTTTTAC TATCCCAATGGGCCGTCTAT 50.0 50.0 58.0 58.0 1042-1117 bp 1.0 88.0
trnK-UUU-rps16_p3 CAGGATCAGTCGCGGTTTTA AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1517-1583 bp 1.0 87.3
trnK-UUU-rps16_p4 AGGATCAGTCGCGGTTTTAC AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.0 58.0 1516-1582 bp 1.0 87.3
trnK-UUU-rps16_p5 CTCATGGGAGAAGCCTTTCC AAAAGGGTATGTTGCTGCCA 55.0 45.0 58.0 58.0 1801-1868 bp 1.0 86.4