Hotspot

ycf1

SSC · 122664-127955 · 5292 bp

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Pi 0.146

MarkerSeek score 68.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5292
variable_sites: 1479
pi: 0.146
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCGGAGTCGATGTGAATCG	CCCACAAAAATGGAGAAATGGA	50.0	40.9	57.8	57.1	609 bp	0.5	35.8
ycf1_p2	TTCGGAGTCGATGTGAATCG	TCCCACAAAAATGGAGAAATGG	50.0	40.9	57.8	57.1	610 bp	0.5	35.8
ycf1_p3	TTCGGAGTCGATGTGAATCG	TCAATCAATCCCATACGTCTCA	50.0	40.9	57.8	57.0	586 bp	0.5	34.7
ycf1_p4	TTCGGAGTCGATGTGAATCG	TGGAATCAATCAATCCCATACGT	50.0	39.1	57.8	58.0	591 bp	0.5	34.5
ycf1_p5	TTCGGAGTCGATGTGAATCG	TGGAAAAACTGGTTAAGAGGTGA	50.0	39.1	57.8	57.9	674 bp	0.5	33.9