Hotspot

psaC

SSC · 116074-116319 · 246 bp

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Pi 0.228

MarkerSeek score 58.40

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 108
pi: 0.228
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	CTCCCGCCGTGTACAAAATA	AAAACTCGGGGAACTCATCG	50.0	50.0	58.0	57.9	987 bp	0.5	59.3
psaC_p2	TCTTTCCTCCCCACATGGAT	AAAACTCGGGGAACTCATCG	50.0	50.0	58.0	57.9	942 bp	0.5	59.3
psaC_p3	CTCCCGCCGTGTACAAAATA	CTCGGGGAACTCATCGATTC	50.0	55.0	58.0	57.9	983 bp	0.5	58.9
psaC_p4	TCTTTCCTCCCCACATGGAT	CTCGGGGAACTCATCGATTC	50.0	55.0	58.0	57.9	938 bp	0.5	58.9
psaC_p5	TTCCAAAGCCACAGGATACG	AAAACTCGGGGAACTCATCG	50.0	50.0	57.9	57.9	1097 bp	0.5	58.6