Hotspot

ndhG

SSC · 117078-117608 · 531 bp

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Pi 0.205

MarkerSeek score 65.20

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 215
pi: 0.205
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CGATGAGTTCCCCGAGTTTT	TTGATCACATCCGAGCGTTT	50.0	45.0	57.9	57.9	1904-1910 bp	0.5	59.0
ndhG_p2	GAATCGATGAGTTCCCCGAG	TTGATCACATCCGAGCGTTT	55.0	45.0	57.9	57.9	1908-1914 bp	0.5	58.6
ndhG_p3	CGATGAGTTCCCCGAGTTTT	TCAAATTGCTTTGGGTCGGT	50.0	45.0	57.9	58.3	1629-1635 bp	0.5	57.4
ndhG_p4	CGATGAGTTCCCCGAGTTTT	AATTGATCACATCCGAGCGT	50.0	45.0	57.9	57.7	1906-1912 bp	0.5	57.2
ndhG_p5	CGATGAGTTCCCCGAGTTTT	ATTGATCACATCCGAGCGTT	50.0	45.0	57.9	57.7	1905-1911 bp	0.5	57.2