Hotspot

ndhF

SSC · 108708-110942 · 2235 bp

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Pi 0.214

MarkerSeek score 79.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 916
pi: 0.214
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CTAGCACTGGGTAGACCTCA	GAGGTAAGATCCGGTGAATCG	55.0	52.4	57.9	58.0	2606 bp	0.5	51.2
ndhF_p2	TCGTTGTGGTCGGACTCTAT	GAGGTAAGATCCGGTGAATCG	50.0	52.4	58.2	58.0	2794 bp	0.5	51.0
ndhF_p3	ATCAATTCGGTCGTTGTGGT	GAGGTAAGATCCGGTGAATCG	45.0	52.4	57.8	58.0	2804 bp	0.5	50.9
ndhF_p4	TCTCTTCCTTCTCCGAGCTC	GAGGTAAGATCCGGTGAATCG	55.0	52.4	58.2	58.0	2736 bp	0.5	50.4
ndhF_p5	CTAGCACTGGGTAGACCTCA	AGGTAAGATCCGGTGAATCG	55.0	50.0	57.9	56.8	2605 bp	0.5	49.9