Hotspot

psaC-ndhE

SSC · 116320-116555 · 236 bp

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Pi 0.229

MarkerSeek score 61.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 236
variable_sites: 108
pi: 0.229
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TCCTCTGTTCTTGGAGCAGA	TAATCGGCGCAATTGCTGTA	50.0	45.0	58.0	58.3	925-930 bp	0.5	57.7
psaC-ndhE_p2	TCCTCTGTTCTTGGAGCAGA	TGGACTACAAAAGCAAGCCA	50.0	45.0	58.0	57.6	1052-1057 bp	0.5	57.6
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	TAATCGGCGCAATTGCTGTA	50.0	45.0	58.1	58.3	887-892 bp	0.5	56.9
psaC-ndhE_p4	TCCTCTGTTCTTGGAGCAGA	CTGAAGCCGCTATTGGACTC	50.0	55.0	58.0	58.4	468 bp	0.5	56.7
psaC-ndhE_p5	TCCTCTGTTCTTGGAGCAGA	CCGCTATTGGACTCGCTATT	50.0	50.0	58.0	57.6	462 bp	0.5	56.5