Hotspot

trnL-UAG-ccsA

SSC · 113095-113236 · 142 bp

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Pi 0.23

MarkerSeek score 59.70

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 142
variable_sites: 62
pi: 0.23
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	556-557 bp	0.5	59.8
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	555-556 bp	0.5	59.8
trnL-UAG-ccsA_p3	GTGAAATCGGTAGACACGCT	TGATCCACAAAGAAGAGCCG	50.0	50.0	58.0	57.9	674-675 bp	0.5	59.6
trnL-UAG-ccsA_p4	TGAAATCGGTAGACACGCTG	TGATCCACAAAGAAGAGCCG	50.0	50.0	58.0	57.9	673-674 bp	0.5	59.6
trnL-UAG-ccsA_p5	TCACTCACATTGTGGTGGTG	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	1201-1202 bp	0.5	59.5