Hotspot

rpl32-trnL-UAG

SSC · 112156-113014 · 859 bp

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Pi 0.208

MarkerSeek score 72.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 859
variable_sites: 341
pi: 0.208
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	991 bp	0.5	57.6
rpl32-trnL-UAG_p2	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	992 bp	0.5	57.6
rpl32-trnL-UAG_p3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.6	58.0	974 bp	0.5	57.4
rpl32-trnL-UAG_p4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.6	58.0	975 bp	0.5	57.4
rpl32-trnL-UAG_p5	AAGGAAAGGATATTGGGCGG	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	57.6	58.1	1527-1528 bp	0.5	56.9