Hotspot

ndhC-trnV-UAC

LSC · 49717-50863 · 1147 bp

Back to Cirsium

Pi 0.00349
MarkerSeek score 61.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1147
variable_sites
23
pi
0.00349
unique_haplotype_count
16
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCCTTTGCTAATCGGAGCTA CCGAGAAAGTCTACGGTTCG 50.0 55.0 57.1 58.1 1273-1294 bp 1.0 75.1
ndhC-trnV-UAC_p2 CCCTTTGCTAATCGGAGCTAA CCGAGAAAGTCTACGGTTCG 47.6 55.0 57.8 58.1 1273-1294 bp 1.0 72.8
ndhC-trnV-UAC_p3 CTCCGGCCCTTTGCTAATC CCGAGAAAGTCTACGGTTCG 57.9 55.0 58.3 58.1 1279-1300 bp 1.0 72.0
ndhC-trnV-UAC_p4 GGCCCTTTGCTAATCGGAG CCGAGAAAGTCTACGGTTCG 57.9 55.0 58.3 58.1 1275-1296 bp 1.0 72.0
ndhC-trnV-UAC_p5 TTCTCCGGCCCTTTGCTAAT CCGAGAAAGTCTACGGTTCG 50.0 55.0 59.4 58.1 1281-1302 bp 1.0 71.3