primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9228	9757	583-598	1.000000	83.0
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9228	9757	601-616	1.000000	80.7
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9228	9757	582-597	1.000000	77.1
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9228	9757	591-606	1.000000	76.9
trnC-GCA-petN_p5	trnC-GCA-petN	5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.063167	9228	9757	640-655	1.000000	76.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	45871	46408	571-597	1.000000	85.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	45871	46408	579-605	1.000000	84.7
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	45871	46408	603-629	1.000000	84.1
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	45871	46408	611-637	1.000000	83.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	45871	46408	585-611	1.000000	83.3
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCCTTTGCTAATCGGAGCTA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.1	58.1	0.0	0.0	1.018220	49717	50863	1273-1294	1.000000	75.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CCCTTTGCTAATCGGAGCTAA	CCGAGAAAGTCTACGGTTCG	21	20	47.6	55.0	57.8	58.1	0.0	0.0	1.301537	49717	50863	1273-1294	1.000000	72.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTCCGGCCCTTTGCTAATC	CCGAGAAAGTCTACGGTTCG	19	20	57.9	55.0	58.3	58.1	0.0	0.0	1.399630	49717	50863	1279-1300	1.000000	72.0
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGCCCTTTGCTAATCGGAG	CCGAGAAAGTCTACGGTTCG	19	20	57.9	55.0	58.3	58.1	0.0	0.0	1.399630	49717	50863	1275-1296	1.000000	72.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TTCTCCGGCCCTTTGCTAAT	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	59.4	58.1	0.0	0.0	1.483809	49717	50863	1281-1302	1.000000	71.3
petA-psbJ_p1	petA-psbJ	1	ACGTGTACAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.0	57.6	17.6	4.1	0.411974	62533	63324	904-935	1.000000	81.7
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.416851	62533	63324	894-925	1.000000	81.7
petA-psbJ_p3	petA-psbJ	3	ACGTGTACAAGGCCTTTTGT	TGTGATCGGTTTAGTAGGCG	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.619192	62533	63324	942-973	1.000000	79.9
petA-psbJ_p4	petA-psbJ	4	GGCCTTTTGTTCTTCTTCGC	TGTGATCGGTTTAGTAGGCG	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.624069	62533	63324	932-963	1.000000	79.9
petA-psbJ_p5	petA-psbJ	5	ACGTGTACAAGGCCTTTTGT	GTGATCGGTTTAGTAGGCGT	20	20	45.0	50.0	58.0	57.4	17.6	0.0	0.620943	62533	63324	941-972	1.000000	79.8
ndhF_p1	ndhF	1	CTAGCACTGGGTAGACCTCA	GAGGTAAGATCCGGTGAATCG	20	21	55.0	52.4	57.9	58.0	0.0	0.0	1.160001	108708	110942	2606-2606	0.500000	51.2
ndhF_p2	ndhF	2	TCGTTGTGGTCGGACTCTAT	GAGGTAAGATCCGGTGAATCG	20	21	50.0	52.4	58.2	58.0	8.2	0.0	1.184325	108708	110942	2794-2794	0.500000	51.0
ndhF_p3	ndhF	3	ATCAATTCGGTCGTTGTGGT	GAGGTAAGATCCGGTGAATCG	20	21	45.0	52.4	57.8	58.0	0.0	0.0	1.193516	108708	110942	2804-2804	0.500000	50.9
ndhF_p4	ndhF	4	TCTCTTCCTTCTCCGAGCTC	GAGGTAAGATCCGGTGAATCG	20	21	55.0	52.4	58.2	58.0	2.7	0.0	1.256922	108708	110942	2736-2736	0.500000	50.4
ndhF_p5	ndhF	5	CTAGCACTGGGTAGACCTCA	AGGTAAGATCCGGTGAATCG	20	20	55.0	50.0	57.9	56.8	0.0	0.0	1.325374	108708	110942	2605-2605	0.500000	49.9
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	CACCACCACAATGTGAGTGA	20	20	50.0	50.0	58.0	58.0	17.5	25.6	0.066479	110943	111990	1554-1556	0.500000	60.0
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	CACCACCACAATGTGAGTGA	20	20	45.0	50.0	58.0	58.0	17.5	25.6	0.077769	110943	111990	1552-1554	0.500000	59.9
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	CACCACCACAATGTGAGTGA	20	20	45.0	50.0	58.0	58.0	17.5	25.6	0.077769	110943	111990	1553-1555	0.500000	59.9
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	AATAAGACTCCCCACCACCA	20	20	50.0	50.0	58.0	57.9	17.5	0.0	0.086161	110943	111990	1566-1568	0.500000	59.9
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	AATAAGACTCCCCACCACCA	20	20	45.0	50.0	58.0	57.9	17.5	0.0	0.097450	110943	111990	1564-1566	0.500000	59.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.372803	112156	113014	991-991	0.500000	57.6
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.374173	112156	113014	992-992	0.500000	57.6
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.400763	112156	113014	974-974	0.500000	57.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.402133	112156	113014	975-975	0.500000	57.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AAGGAAAGGATATTGGGCGG	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.453915	112156	113014	1527-1528	0.500000	56.9
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	GTGAAATCGGTAGACACGCT	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.096773	113095	113236	556-557	0.500000	59.8
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	TGAAATCGGTAGACACGCTG	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.098143	113095	113236	555-556	0.500000	59.8
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	GTGAAATCGGTAGACACGCT	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.106972	113095	113236	674-675	0.500000	59.6
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	TGAAATCGGTAGACACGCTG	TGATCCACAAAGAAGAGCCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.108342	113095	113236	673-674	0.500000	59.6
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	TCACTCACATTGTGGTGGTG	GAAGTAGCAAAGCCTTGGGT	20	20	50.0	50.0	58.0	58.1	25.1	0.0	0.123008	113095	113236	1201-1202	0.500000	59.5
ccsA_p1	ccsA	1	GTGAAATCGGTAGACACGCT	AGAACCGCGTGAATCACTAC	20	20	50.0	50.0	58.0	58.0	0.0	11.9	0.015661	113237	114205	1296-1297	0.500000	60.3
ccsA_p2	ccsA	2	TGAAATCGGTAGACACGCTG	AGAACCGCGTGAATCACTAC	20	20	50.0	50.0	58.0	58.0	0.0	11.9	0.017031	113237	114205	1295-1296	0.500000	60.3
ccsA_p3	ccsA	3	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044083	113237	114205	1571-1572	0.500000	60.0
ccsA_p4	ccsA	4	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045454	113237	114205	1570-1571	0.500000	60.0
ccsA_p5	ccsA	5	GTGAAATCGGTAGACACGCT	TAGCTTTTCGATGGCTTCCC	20	20	50.0	50.0	58.0	58.0	0.0	11.4	0.046217	113237	114205	1794-1795	0.500000	59.9
ndhD_p1	ndhD	1	GTAGTGATTCACGCGGTTCT	TCTGCTCCAAGAACAGAGGA	20	20	50.0	50.0	58.0	58.0	12.5	0.0	0.023287	114452	115972	1899-1899	0.500000	60.0
ndhD_p2	ndhD	2	AGCAAATTCCGCAATTGTGG	TCTGCTCCAAGAACAGAGGA	20	20	45.0	50.0	57.9	58.0	28.7	0.0	0.098623	114452	115972	2115-2115	0.500000	59.4
ndhD_p3	ndhD	3	GTAGTGATTCACGCGGTTCT	AAAACTCGGGGAACTCATCG	20	20	50.0	50.0	58.0	57.9	12.5	0.0	0.102592	114452	115972	2121-2121	0.500000	59.3
ndhD_p4	ndhD	4	GTAGTGATTCACGCGGTTCT	TGATACCTTGGGACGGATGT	20	20	50.0	50.0	58.0	58.1	12.5	0.0	0.133701	114452	115972	1937-1937	0.500000	59.1
ndhD_p5	ndhD	5	GTAGTGATTCACGCGGTTCT	CTCGGGGAACTCATCGATTC	20	20	50.0	55.0	58.0	57.9	12.5	0.0	0.149365	114452	115972	2117-2117	0.500000	59.0
psaC_p1	psaC	1	CTCCCGCCGTGTACAAAATA	AAAACTCGGGGAACTCATCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.107699	116074	116319	987-987	0.500000	59.3
psaC_p2	psaC	2	TCTTTCCTCCCCACATGGAT	AAAACTCGGGGAACTCATCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.109141	116074	116319	942-942	0.500000	59.3
psaC_p3	psaC	3	CTCCCGCCGTGTACAAAATA	CTCGGGGAACTCATCGATTC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.154472	116074	116319	983-983	0.500000	58.9
psaC_p4	psaC	4	TCTTTCCTCCCCACATGGAT	CTCGGGGAACTCATCGATTC	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.155914	116074	116319	938-938	0.500000	58.9
psaC_p5	psaC	5	TTCCAAAGCCACAGGATACG	AAAACTCGGGGAACTCATCG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.202712	116074	116319	1097-1097	0.500000	58.6
psaC-ndhE_p1	psaC-ndhE	1	TCCTCTGTTCTTGGAGCAGA	TAATCGGCGCAATTGCTGTA	20	20	50.0	45.0	58.0	58.3	12.1	26.9	0.357634	116320	116555	925-930	0.500000	57.7
psaC-ndhE_p2	psaC-ndhE	2	TCCTCTGTTCTTGGAGCAGA	TGGACTACAAAAGCAAGCCA	20	20	50.0	45.0	58.0	57.6	12.1	0.0	0.372121	116320	116555	1052-1057	0.500000	57.6
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	TAATCGGCGCAATTGCTGTA	20	20	50.0	45.0	58.1	58.3	0.0	26.9	0.468048	116320	116555	887-892	0.500000	56.9
psaC-ndhE_p4	psaC-ndhE	4	TCCTCTGTTCTTGGAGCAGA	CTGAAGCCGCTATTGGACTC	20	20	50.0	55.0	58.0	58.4	12.1	0.0	0.439101	116320	116555	468-468	0.500000	56.7
psaC-ndhE_p5	psaC-ndhE	5	TCCTCTGTTCTTGGAGCAGA	CCGCTATTGGACTCGCTATT	20	20	50.0	50.0	58.0	57.6	12.1	0.0	0.455995	116320	116555	462-462	0.500000	56.5
ndhG_p1	ndhG	1	CGATGAGTTCCCCGAGTTTT	TTGATCACATCCGAGCGTTT	20	20	50.0	45.0	57.9	57.9	0.0	0.0	0.188068	117078	117608	1904-1910	0.500000	59.0
ndhG_p2	ndhG	2	GAATCGATGAGTTCCCCGAG	TTGATCACATCCGAGCGTTT	20	20	55.0	45.0	57.9	57.9	0.0	0.0	0.234841	117078	117608	1908-1914	0.500000	58.6
ndhG_p3	ndhG	3	CGATGAGTTCCCCGAGTTTT	TCAAATTGCTTTGGGTCGGT	20	20	50.0	45.0	57.9	58.3	0.0	0.0	0.396256	117078	117608	1629-1635	0.500000	57.4
ndhG_p4	ndhG	4	CGATGAGTTCCCCGAGTTTT	AATTGATCACATCCGAGCGT	20	20	50.0	45.0	57.9	57.7	0.0	0.0	0.410687	117078	117608	1906-1912	0.500000	57.2
ndhG_p5	ndhG	5	CGATGAGTTCCCCGAGTTTT	ATTGATCACATCCGAGCGTT	20	20	50.0	45.0	57.9	57.7	0.0	0.0	0.410687	117078	117608	1905-1911	0.500000	57.2
ndhA_p1	ndhA	1	TGACCAATATACCTTGCGGC	GGCAAAAAGAGGGGGATTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101015	118522	120671	2721-2750	0.500000	59.7
ndhA_p2	ndhA	2	TGACCAATATACCTTGCGGC	TGGCAAAAAGAGGGGGATTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.101015	118522	120671	2722-2751	0.500000	59.7
ndhA_p3	ndhA	3	TGACCAATATACCTTGCGGC	TGGGAAGTCCAATGGCAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.132307	118522	120671	2734-2763	0.500000	59.4
ndhA_p4	ndhA	4	TGACCAATATACCTTGCGGC	TTAGAAATCCGCCGCTTTGA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.140276	118522	120671	2605-2634	0.500000	59.4
ndhA_p5	ndhA	5	AAACGCTCGGATGTGATCAA	GGCAAAAAGAGGGGGATTCA	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.156115	118522	120671	2808-2837	0.500000	59.2
ndhH_p1	ndhH	1	CGTTGTTGTATACCCGCAGA	TCAACGGTTGCTGGCTTATT	20	20	50.0	45.0	58.0	58.1	0.0	15.9	0.096665	120673	121854	1550-1556	0.500000	59.7
ndhH_p2	ndhH	2	TGCGTATTCAGGGCCAATAC	TCAACGGTTGCTGGCTTATT	20	20	50.0	45.0	58.0	58.1	6.2	15.9	0.133920	120673	121854	1569-1575	0.500000	59.4
ndhH_p3	ndhH	3	CGTTGTTGTATACCCGCAGA	TCTTCTGTCCGGCATCTGTA	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.153623	120673	121854	2108-2117	0.500000	59.3
ndhH_p4	ndhH	4	CCTGACATAAGAAGGCCGAC	TCAACGGTTGCTGGCTTATT	20	20	55.0	45.0	58.1	58.1	0.0	15.9	0.154388	120673	121854	1814-1820	0.500000	59.2
ndhH_p5	ndhH	5	GACATAAGAAGGCCGACAGG	TCAACGGTTGCTGGCTTATT	20	20	55.0	45.0	58.1	58.1	0.0	15.9	0.154388	120673	121854	1811-1817	0.500000	59.2
rps15_p1	rps15	1	TCCCAACGCGTTACATAAGG	AATAATGGGAGCCGCTTGAG	20	20	50.0	50.0	58.0	58.0	26.1	0.0	0.040065	121952	122230	1062-1071	0.500000	60.5
rps15_p2	rps15	2	AACACCATGCATTGATGGGT	AATAATGGGAGCCGCTTGAG	20	20	45.0	50.0	58.1	58.0	8.9	0.0	0.085751	121952	122230	925-934	0.500000	60.3
rps15_p3	rps15	3	TCCCAACGCGTTACATAAGG	TTTCTTCTGTCCGGCATCTG	20	20	50.0	50.0	58.0	57.9	26.1	0.0	0.112078	121952	122230	957-966	0.500000	60.0
rps15_p4	rps15	4	GCAGTGACTCCTCCAATACG	AATAATGGGAGCCGCTTGAG	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.099233	121952	122230	1344-1353	0.500000	59.9
rps15_p5	rps15	5	AGGAGTCTGTGCCCCAATAT	AATAATGGGAGCCGCTTGAG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.128265	121952	122230	1240-1249	0.500000	59.7
rps15-ycf1_p1	rps15-ycf1	1	TCCCAACGCGTTACATAAGG	AATAATGGGAGCCGCTTGAG	20	20	50.0	50.0	58.0	58.0	26.1	0.0	0.040065	122231	122663	1062-1071	0.500000	60.5
rps15-ycf1_p2	rps15-ycf1	2	AACACCATGCATTGATGGGT	AATAATGGGAGCCGCTTGAG	20	20	45.0	50.0	58.1	58.0	8.9	0.0	0.085751	122231	122663	925-934	0.500000	60.3
rps15-ycf1_p3	rps15-ycf1	3	CCGCAGACCTCTCTGAGATA	AATAATGGGAGCCGCTTGAG	20	20	55.0	50.0	58.0	58.0	8.0	0.0	0.052863	122231	122663	648-651	0.500000	60.1
rps15-ycf1_p4	rps15-ycf1	4	AATAAGCCAGCAACCGTTGA	AATAATGGGAGCCGCTTGAG	20	20	45.0	50.0	58.1	58.0	15.6	0.0	0.122841	122231	122663	685-688	0.500000	59.5
rps15-ycf1_p5	rps15-ycf1	5	CCAGAATTTTCCGCAGACCT	AATAATGGGAGCCGCTTGAG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.200743	122231	122663	658-661	0.444444	56.5
ycf1_p1	ycf1	1	TTCGGAGTCGATGTGAATCG	CCCACAAAAATGGAGAAATGGA	20	22	50.0	40.9	57.8	57.1	1.8	0.0	3.077163	123768	123887	609-609	0.500000	35.8
ycf1_p2	ycf1	2	TTCGGAGTCGATGTGAATCG	TCCCACAAAAATGGAGAAATGG	20	22	50.0	40.9	57.8	57.1	1.8	0.0	3.077163	123768	123887	610-610	0.500000	35.8
ycf1_p3	ycf1	3	TTCGGAGTCGATGTGAATCG	TCAATCAATCCCATACGTCTCA	20	22	50.0	40.9	57.8	57.0	1.8	0.0	3.208365	123768	123887	586-586	0.500000	34.7
ycf1_p4	ycf1	4	TTCGGAGTCGATGTGAATCG	TGGAATCAATCAATCCCATACGT	20	23	50.0	39.1	57.8	58.0	1.8	0.0	3.236403	123768	123887	591-591	0.500000	34.5
ycf1_p5	ycf1	5	TTCGGAGTCGATGTGAATCG	TGGAAAAACTGGTTAAGAGGTGA	20	23	50.0	39.1	57.8	57.9	1.8	0.0	3.307566	123768	123887	674-674	0.500000	33.9