Hotspot

rpl16

LSC · 84596-86039 · 1444 bp

Back to Circaea

Pi 0.00571

MarkerSeek score 49.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1444
variable_sites: 26
pi: 0.00571
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTCAATTCTCGGGCAATCG	AAGGTCGGGTTCCTCTACAA	50.0	50.0	58.1	58.0	2173-2200 bp	1.0	81.2
rpl16_p2	TCTCAATTCTCGGGCAATCG	AGGTCGGGTTCCTCTACAAA	50.0	50.0	58.1	58.0	2172-2199 bp	1.0	81.2
rpl16_p3	TCTCAATTCTCGGGCAATCG	CAAGTACAAATTGCAGGGCG	50.0	50.0	58.1	58.0	2234-2261 bp	1.0	81.1
rpl16_p4	CTGTCTCAATTCTCGGGCAA	CAAGTACAAATTGCAGGGCG	50.0	50.0	57.9	58.0	2237-2264 bp	1.0	80.8
rpl16_p5	CTGTCTCAATTCTCGGGCAA	AGGTCGGGTTCCTCTACAAA	50.0	50.0	57.9	58.0	2175-2202 bp	1.0	80.8