Hotspot

ndhF

SSC · 112682-114922 · 2241 bp

Back to Circaea

Pi 0.00599

MarkerSeek score 47.40

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 44
pi: 0.00599
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GAGCTACTGAGGAACAACGG	AGAGCCGGTGATTCAGAAAC	55.0	50.0	58.0	57.9	2856-2857 bp	1.0	80.5
ndhF_p2	CCGGAACTTCTTCGTAGTGG	AGAGCCGGTGATTCAGAAAC	55.0	50.0	58.0	57.9	2760-2761 bp	1.0	80.5
ndhF_p3	AATTCGGTCGTTATGGTCGG	CATGGGCTTTCCTGGCATAT	50.0	50.0	58.1	58.0	2762-2768 bp	0.857143	74.7
ndhF_p4	AATTCGGTCGTTATGGTCGG	TTCAAAAAGCGAGTCTGCCT	50.0	45.0	58.1	58.0	2897-2940 bp	0.714286	69.6
ndhF_p5	AATTCGGTCGTTATGGTCGG	GTTCAAAAAGCGAGTCTGCC	50.0	50.0	58.1	58.0	2898-2941 bp	0.714286	69.5