Hotspot

petA-psbJ

LSC · 65784-66786 · 1003 bp

Back to Circaea

Pi 0.0124

MarkerSeek score 68.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1003
variable_sites: 40
pi: 0.0124
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	TTCATACTCCGGGTTAGGCT	50.0	50.0	58.0	57.8	1151-1161 bp	1.0	81.2
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	AGCCGGTATTCTTGTGATCG	50.0	50.0	58.0	57.8	1196-1206 bp	1.0	80.7
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	GTTCATACTCCGGGTTAGGC	50.0	55.0	58.0	57.8	1152-1162 bp	1.0	80.7
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	TTCATACTCCGGGTTAGGCT	50.0	50.0	57.8	57.8	1134-1144 bp	1.0	80.4
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	TACTCCGGGTTAGGCTCATC	50.0	55.0	58.0	58.3	1147-1157 bp	1.0	80.2