Hotspot

trnT-UGU-trnL-UAA

LSC · 48384-49486 · 1103 bp

Back to Circaea

Pi 0.00964

MarkerSeek score 53.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1103
variable_sites: 32
pi: 0.00964
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	CGAAGCCATACCCAATCGAA	TTCTAAACCCCCGTTGTTGG	50.0	50.0	58.1	58.0	1994-2012 bp	1.0	82.0
trnT-UGU-trnL-UAA_p2	AAAACCTGACCTGTTGACCC	TTCTAAACCCCCGTTGTTGG	50.0	50.0	57.9	58.0	1936-1954 bp	1.0	81.9
trnT-UGU-trnL-UAA_p3	CGAAGCCATACCCAATCGAA	AGTCTCTGCACCTACCCTTT	50.0	50.0	58.1	58.0	2024-2042 bp	1.0	81.9
trnT-UGU-trnL-UAA_p4	CGAAGCCATACCCAATCGAA	AAAGAGATTCGCGCCATCAT	50.0	45.0	58.1	58.0	2189-2207 bp	1.0	81.6
trnT-UGU-trnL-UAA_p5	CGAAGCCATACCCAATCGAA	AAGAGATTCGCGCCATCATT	50.0	45.0	58.1	58.0	2188-2206 bp	1.0	81.6