Hotspot

ycf3-trnS-GGA

LSC · 46036-46944 · 909 bp

Back to Circaea

Pi 0.00771

MarkerSeek score 63.20

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 909
variable_sites: 23
pi: 0.00771
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CGGCGACAATTGAAAAGGTC	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.0	57.8	987-1032 bp	1.0	83.3
ycf3-trnS-GGA_p2	TTTTCCCCTGAAGTTGTCGG	TTCCAATGCTACGCCTTGAA	50.0	45.0	58.0	57.8	1027-1072 bp	1.0	83.2
ycf3-trnS-GGA_p3	CGGCGACAATTGAAAAGGTC	TTAGTTGAGGGTGCGGAAAG	50.0	50.0	58.0	57.8	1050-1095 bp	1.0	83.1
ycf3-trnS-GGA_p4	TTTTCCCCTGAAGTTGTCGG	TTAGTTGAGGGTGCGGAAAG	50.0	50.0	58.0	57.8	1090-1135 bp	1.0	83.0
ycf3-trnS-GGA_p5	TTTTCCCCTGAAGTTGTCGG	GTGCGGAAAGAGAGGGATTC	50.0	55.0	58.0	58.3	1080-1125 bp	1.0	81.7