Hotspot

psaA-ycf3

LSC · 43270-44020 · 751 bp

Back to Circaea

Pi 0.0162

MarkerSeek score 62.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 32
pi: 0.0162
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	ACCTGAGCACTAGGTCCAAT	CTGAGCCGTATGAGGTAGGA	50.0	55.0	58.0	58.0	1273-1317 bp	1.0	84.6
psaA-ycf3_p2	ATTTGGGAGCAGCTTTGTGA	CTGAGCCGTATGAGGTAGGA	45.0	55.0	58.0	58.0	1485-1529 bp	1.0	84.3
psaA-ycf3_p3	TTTGGGAGCAGCTTTGTGAT	CTGAGCCGTATGAGGTAGGA	45.0	55.0	58.0	58.0	1484-1528 bp	1.0	84.3
psaA-ycf3_p4	ATTTGGGAGCAGCTTTGTGA	CTAAGGAATTGAGCAGCGGT	45.0	50.0	58.0	58.0	1842-1891 bp	1.0	84.0
psaA-ycf3_p5	TTTGGGAGCAGCTTTGTGAT	CTAAGGAATTGAGCAGCGGT	45.0	50.0	58.0	58.0	1841-1890 bp	1.0	84.0