Hotspot

rpoB-trnC-GCA

LSC · 27718-28978 · 1261 bp

Back to Circaea

Pi 0.00782

MarkerSeek score 57.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1261
variable_sites: 31
pi: 0.00782
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTCCCCCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.3	58.1	1340-1366 bp	1.0	79.5
rpoB-trnC-GCA_p2	CATTCCCCCATTTCCATCCC	TTGTTAATGAGGCGACACCC	55.0	50.0	58.3	58.2	1358-1384 bp	1.0	78.7
rpoB-trnC-GCA_p3	ACATTCCCCCATTTCCATCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1341-1367 bp	1.0	77.9
rpoB-trnC-GCA_p4	CATTCCCCCATTTCCATCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.3	58.4	1322-1348 bp	1.0	77.4
rpoB-trnC-GCA_p5	ACATTCCCCCATTTCCATCC	TTGTTAATGAGGCGACACCC	50.0	50.0	57.5	58.2	1359-1385 bp	1.0	77.1