Hotspot

atpH-atpI

LSC · 14557-15450 · 894 bp

Back to Circaea

Pi 0.00724

MarkerSeek score 66.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 894
variable_sites: 21
pi: 0.00724
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GAAGCAAGTCCGACTGCTAA	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	973-982 bp	1.0	80.3
atpH-atpI_p2	GGCAGAAACCAGTGGATTCA	CGAATCCATGGAGGGTCATC	50.0	55.0	58.1	57.8	932-941 bp	1.0	80.3
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	999-1008 bp	1.0	80.0
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	944-953 bp	1.0	79.6
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.2	57.8	1003-1012 bp	1.0	79.4