Hotspot

trnR-UCU-atpA

LSC · 10422-10899 · 478 bp

Back to Circaea

Pi 0.012

MarkerSeek score 57.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 18
pi: 0.012
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CAGGAACAGAAGGAACGCTT	47.6	50.0	57.3	58.1	527-541 bp	1.0	69.6
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TTCAGGAACAGAAGGAACGC	47.6	50.0	57.3	58.1	529-543 bp	1.0	69.5
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	ACGACGCTATTCAGGAACAG	47.6	50.0	57.3	57.7	538-552 bp	1.0	68.2
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	CGACGCTATTCAGGAACAGA	47.6	50.0	57.3	57.4	537-551 bp	1.0	65.9
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	AGGAACAGAAGGAACGCTTT	47.6	45.0	57.3	57.4	526-540 bp	1.0	65.4