Hotspot

psbI-trnS-GCU

LSC · 8353-8473 · 121 bp

Back to Circaea

Pi 0.0335

MarkerSeek score 58.20

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 10
pi: 0.0335
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CAAGCTGCTGTCAGTTTTCG	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.0	58.0	688-722 bp	1.0	82.3
psbI-trnS-GCU_p2	CAAGCTGCTGTCAGTTTTCG	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.0	58.0	689-723 bp	1.0	82.3
psbI-trnS-GCU_p3	AAGCTGCTGTCAGTTTTCGA	TGGACTAAAGCGTCGGATTG	45.0	50.0	58.0	58.0	687-721 bp	1.0	82.2
psbI-trnS-GCU_p4	AAGCTGCTGTCAGTTTTCGA	GTGGACTAAAGCGTCGGATT	45.0	50.0	58.0	58.0	688-722 bp	1.0	82.2
psbI-trnS-GCU_p5	TCGCCCCATTTACTCATTCG	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.1	58.0	529-551 bp	1.0	82.2