Hotspot

rps16-trnQ-UUG

LSC · 6415-7268 · 854 bp

Back to Circaea

Pi 0.00736

MarkerSeek score 55.80

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 854
variable_sites: 20
pi: 0.00736
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGCGTTGCTTTTTACCACAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	921-923 bp	1.0	80.9
rps16-trnQ-UUG_p2	ATCATGTCTGTCAAGTCGCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	939-941 bp	1.0	78.2
rps16-trnQ-UUG_p3	CGCGTTGCTTTTTACCACAT	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.0	57.3	925-927 bp	1.0	76.1
rps16-trnQ-UUG_p4	CGCGTTGCTTTTTACCACAT	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.0	58.7	951-953 bp	1.0	76.0
rps16-trnQ-UUG_p5	CGCGTTGCTTTTTACCACAT	GGGTTTTGGTCCCGCTATTC	45.0	55.0	58.0	58.9	942-944 bp	1.0	74.2