Hotspot

trnK-UUU-rps16

LSC · 4340-5292 · 953 bp

Back to Circaea

Pi 0.0112
MarkerSeek score 57.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
953
variable_sites
34
pi
0.0112
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAGCGGGGGTTTTTACAGA 50.0 45.0 58.2 57.6 1033-1061 bp 1.0 78.9
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT AAGCGGGGGTTTTTACAGAA 50.0 45.0 58.2 57.6 1032-1060 bp 1.0 78.9
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT AAAGCGGGGGTTTTTACAGA 50.0 45.0 58.2 57.6 1032-1060 bp 1.0 78.9
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AAGCGGGGGTTTTTACAGAA 50.0 45.0 58.2 57.6 1031-1059 bp 1.0 78.9
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA AAGCGGGGGTTTTTACAGAA 50.0 45.0 58.3 57.6 1041-1069 bp 1.0 78.0