Hotspot

ycf2

Genome · 89905-96876 · 6972 bp

Back to Cinnamomum

Pi 0.00261

MarkerSeek score 37.90

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6972
variable_sites: 88
pi: 0.00261
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TCACCAATTGAGTCACAGGT	ACGGAGATAGTGATGCAAGT	45.0	45.0	56.7	56.3	261 bp	1.0	61.1
ycf2_p2	TTGAGTCACAGGTATCTGGC	ACGGAGATAGTGATGCAAGT	50.0	45.0	56.9	56.3	254 bp	0.967742	61.1
ycf2_p3	TTGAGTCACAGGTATCTGGC	TGAAACGGAGATAGTGATGCA	50.0	42.9	56.9	57.1	258 bp	0.967742	59.2
ycf2_p4	TTGAGTCACAGGTATCTGGC	AACGGAGATAGTGATGCAAGT	50.0	42.9	56.9	57.1	255 bp	0.967742	59.2
ycf2_p5	TTGAGTCACAGGTATCTGGC	ACGGAGATAGTGATGCAAGTT	50.0	42.9	56.9	57.1	254 bp	0.967742	59.2