Hotspot

rpl32-trnL-UAG

Genome · 117389-118773 · 1385 bp

Back to Cinnamomum

Pi 0.0086

MarkerSeek score 60.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 1385
variable_sites: 56
pi: 0.0086
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGCGGCAAAAGCTTTATCTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.7	57.9	1517-1525 bp	1.0	81.1
rpl32-trnL-UAG_p2	CGCGGCAAAAGCTTTATCTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.7	57.8	1510-1518 bp	1.0	80.6
rpl32-trnL-UAG_p3	CGCGGCAAAAGCTTTATCTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.7	57.8	1516-1524 bp	1.0	80.5
rpl32-trnL-UAG_p4	CGCGGCAAAAGCTTTATCTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.7	57.8	1515-1523 bp	1.0	80.5
rpl32-trnL-UAG_p5	CGCGGCAAAAGCTTTATCTT	GAGCAGCGTGTCTACCAATT	45.0	50.0	57.7	58.3	1511-1519 bp	1.0	79.9