Hotspot

ndhF-rpl32

Genome · 116093-117214 · 1122 bp

Back to Cinnamomum

Pi 0.00766

MarkerSeek score 61.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: Genome
length_bp: 1122
variable_sites: 37
pi: 0.00766
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAACAAGCAGAAGTCCCAA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1234-1347 bp	1.0	81.2
ndhF-rpl32_p2	AGCAGAAGTCCCAACCCTAT	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.0	57.7	1228-1341 bp	1.0	81.1
ndhF-rpl32_p3	AAGCAGAAGTCCCAACCCTA	GTACGTTTCTTTGGAACCGC	50.0	50.0	58.3	57.7	1229-1342 bp	1.0	79.3
ndhF-rpl32_p4	GGAACAAGCAGAAGTCCCAA	GGCCTGATATCCCCTTCCTT	50.0	55.0	58.0	58.5	1292-1405 bp	1.0	79.1
ndhF-rpl32_p5	AGCAGAAGTCCCAACCCTAT	GGCCTGATATCCCCTTCCTT	50.0	55.0	58.0	58.5	1286-1399 bp	1.0	79.0