Hotspot

ycf4-cemA

Genome · 63557-64467 · 911 bp

Back to Cinnamomum

Pi 0.00247

MarkerSeek score 52.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: Genome
length_bp: 911
variable_sites: 9
pi: 0.00247
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	AGATCCACCAGGGCAAAAAG	55.0	50.0	58.0	58.1	1060-1088 bp	1.0	82.2
ycf4-cemA_p2	CATTCCCTTGACCCGTACTG	GATCCACCAGGGCAAAAAGA	55.0	50.0	58.0	58.1	1059-1087 bp	1.0	82.2
ycf4-cemA_p3	CATTCCCTTGACCCGTACTG	GAGATCCACCAGGGCAAAAA	55.0	50.0	58.0	58.1	1061-1089 bp	1.0	82.1
ycf4-cemA_p4	ATTCCCTTGACCCGTACTGA	AGATCCACCAGGGCAAAAAG	50.0	50.0	58.1	58.1	1059-1087 bp	1.0	82.0
ycf4-cemA_p5	ATTCCCTTGACCCGTACTGA	GATCCACCAGGGCAAAAAGA	50.0	50.0	58.1	58.1	1058-1086 bp	1.0	81.9