Hotspot

psaA-ycf3

Genome · 44057-44695 · 639 bp

Back to Cinnamomum

Pi 0.000868

MarkerSeek score 50.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: Genome
length_bp: 639
variable_sites: 4
pi: 0.000868
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TGGTTGAAGATCGCAAGGAG	50.0	50.0	57.9	57.9	663-751 bp	1.0	82.7
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GTTGAAGATCGCAAGGAGGT	50.0	50.0	57.9	57.9	661-749 bp	1.0	82.7
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	GTCCGATCAAGCTGCTGAAT	50.0	50.0	57.9	58.3	736-824 bp	1.0	80.8
psaA-ycf3_p4	TGTTCTTGAGAAATGGCCGG	TGGTTGAAGATCGCAAGGAG	50.0	50.0	58.5	57.9	688-776 bp	1.0	79.9
psaA-ycf3_p5	TGTTCTTGAGAAATGGCCGG	GTTGAAGATCGCAAGGAGGT	50.0	50.0	58.5	57.9	686-774 bp	1.0	79.9