Hotspot

trnC-GCA-petN

Genome · 29043-30098 · 1056 bp

Back to Cinnamomum

Pi 0.00405

MarkerSeek score 47.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: Genome
length_bp: 1056
variable_sites: 16
pi: 0.00405
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.9	58.2	1134-1170 bp	1.0	82.3
trnC-GCA-petN_p2	CTGCAAATCCTTTCTCCCCA	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.8	58.2	1090-1126 bp	1.0	81.2
trnC-GCA-petN_p3	TGCAAATCCTTTCTCCCCAG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.8	58.2	1089-1125 bp	1.0	81.2
trnC-GCA-petN_p4	GCAAATCCTTTCTCCCCAGT	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.8	58.2	1088-1124 bp	1.0	81.2
trnC-GCA-petN_p5	ACTGCAAATCCTTTCTCCCC	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.8	58.2	1091-1127 bp	1.0	81.1