Hotspot

rbcL

LSC · 56696-58132 · 1437 bp

Back to Cinchona

Pi 0.00162

MarkerSeek score 59.80

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1437
variable_sites: 4
pi: 0.00162
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.1	58.4	1504 bp	1.0	69.7
rbcL_p2	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.1	58.4	1505 bp	1.0	69.7
rbcL_p3	ACAGCTAATTCGTGTCGAGT	TGGGCCGAGTTTAATTGCAA	45.0	45.0	57.0	58.4	1552 bp	1.0	69.1
rbcL_p4	ACAGCTAATTCGTGTCGAGT	TTGGGCCGAGTTTAATTGCA	45.0	45.0	57.0	58.4	1553 bp	1.0	69.1
rbcL_p5	TTCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	47.6	45.0	57.8	58.4	1505 bp	1.0	67.6