Hotspot

petL-petG

LSC · 67645-67820 · 176 bp

Back to Cinchona

Pi 0.0104

MarkerSeek score 57.70

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 176
variable_sites: 3
pi: 0.0104
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TGGTTTGAGCAAGATACGACT	TGATCACCACGCCTGTATTG	42.9	50.0	57.3	58.0	304-305 bp	1.0	67.4
petL-petG_p2	TGGTTTGAGCAAGATACGACT	GATCACCACGCCTGTATTGT	42.9	50.0	57.3	58.0	303-304 bp	1.0	67.4
petL-petG_p3	TGGTTTGAGCAAGATACGACT	CTGATCACCACGCCTGTATT	42.9	50.0	57.3	57.7	305-306 bp	1.0	65.1
petL-petG_p4	TGGTTTGAGCAAGATACGACTT	TGATCACCACGCCTGTATTG	40.9	50.0	58.0	58.0	304-305 bp	1.0	64.8
petL-petG_p5	TTGGTTTGAGCAAGATACGACT	TGATCACCACGCCTGTATTG	40.9	50.0	58.0	58.0	305-306 bp	1.0	64.8