Hotspot

psbZ-trnG-GCC

LSC · 37240-37559 · 320 bp

Back to Cinchona

Pi 0.00313

MarkerSeek score 59.30

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 320
variable_sites: 2
pi: 0.00313
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	462-477 bp	1.0	79.9
psbZ-trnG-GCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	460-475 bp	1.0	79.5
psbZ-trnG-GCC_p3	TTGCTTCTCCTGATGGTTGG	TACCATTCGACCATATCCGC	50.0	50.0	57.8	57.3	435-450 bp	1.0	75.5
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	TACCATTCGACCATATCCGC	55.0	50.0	58.3	57.3	433-448 bp	1.0	75.2
psbZ-trnG-GCC_p5	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	456-471 bp	1.0	74.6