Hotspot

trnT-GGU-psbD

LSC · 32730-33976 · 1247 bp

Back to Cinchona

Pi 0.01
MarkerSeek score 62.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1247
variable_sites
21
pi
0.01
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTACGTAACC 55.0 55.0 58.1 58.0 1389-1396 bp 1.0 81.5
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1392-1399 bp 1.0 81.3
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1407-1414 bp 1.0 80.6
trnT-GGU-psbD_p4 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1410-1417 bp 1.0 80.4
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GAAACGGTCCCTACGTAACC 50.0 55.0 58.3 58.0 1366-1373 bp 1.0 79.8