Hotspot

trnC-GCA-petN

LSC · 28395-29274 · 880 bp

Back to Cinchona

Pi 0.0121

MarkerSeek score 60.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 19
pi: 0.0121
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TGTATATCGTTTTGGCGGCA	ACTTCTTCCCCACACTACGA	45.0	50.0	58.0	58.0	1034-1045 bp	1.0	82.1
trnC-GCA-petN_p2	TGTATATCGTTTTGGCGGCA	TTCTTCCCCACACTACGAGT	45.0	50.0	58.0	58.0	1032-1043 bp	1.0	82.1
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	ACTTCTTCCCCACACTACGA	50.0	50.0	58.1	58.0	979-990 bp	1.0	81.7
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TTCTTCCCCACACTACGAGT	50.0	50.0	58.1	58.0	977-988 bp	1.0	81.7
trnC-GCA-petN_p5	TGTATATCGTTTTGGCGGCA	CACTTCTTCCCCACACTACG	45.0	55.0	58.0	57.9	1035-1046 bp	1.0	81.5