Hotspot

trnG-GCC-trnR-UCU

LSC · 10145-10317 · 173 bp

Back to Cinchona

Pi 0.00963
MarkerSeek score 59.50
Primer available yes
Label trnG-GCC-trnR-UCU

Feature Metadata

label_name
trnG-GCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
173
variable_sites
3
pi
0.00963
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 251-252 bp 1.0 64.6
trnG-GCC-trnR-UCU_p2 ACCCTTAGCCTTCCAAGCTA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 257-258 bp 1.0 64.6
trnG-GCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 257-258 bp 1.0 63.9
trnG-GCC-trnR-UCU_p4 ACCCTTAGCCTTCCAAGCTA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 263-264 bp 1.0 63.9
trnG-GCC-trnR-UCU_p5 TCGACGTCGACTATAACCCT AGAAGACCTCTGTCCTATCCA 50.0 47.6 57.7 56.9 272-273 bp 1.0 62.2