Hotspot

psbK-psbI

LSC · 8159-8573 · 415 bp

Back to Cinchona

Pi 0.0116

MarkerSeek score 63.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 415
variable_sites: 9
pi: 0.0116
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.1	57.6	558-565 bp	1.0	78.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	553-560 bp	1.0	78.4
psbK-psbI_p3	ATGCCAGTCATACCTGTCCT	TTCTTCACGCCCAGGATTAC	50.0	50.0	58.1	57.6	592-599 bp	1.0	77.8
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	550-557 bp	1.0	76.4
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	551-558 bp	1.0	76.4