Hotspot

rps16

LSC · 22751-23878 · 1128 bp

Back to Cichorium

Pi 0.00268

MarkerSeek score 60.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1128
variable_sites: 3
pi: 0.00268
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAGCAAGAAAAGAAGGAAA	TCTCTGTACCGACTGAACCA	35.0	50.0	52.6	57.7	1297-1310 bp	1.0	40.6
rps16_p2	AGAGCAAGAAAAGAAGGAAAT	TCTCTGTACCGACTGAACCA	33.3	50.0	53.3	57.7	1297-1310 bp	1.0	40.6
rps16_p3	AGAGCAAGAAAAGAAGGAAA	GTCTCTGTACCGACTGAACC	35.0	55.0	52.6	57.4	1298-1311 bp	1.0	40.6
rps16_p4	AGAAATCACTATCCTACCCTTAGT	TCTCTGTACCGACTGAACCA	37.5	50.0	56.1	57.7	1238-1251 bp	1.0	40.6
rps16_p5	AGAAATCACTATCCTACCCT	TCTCTGTACCGACTGAACCA	40.0	50.0	52.0	57.7	1238-1251 bp	1.0	40.6