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psbE-petL

LSC · 82182-83466 · 1285 bp

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Pi 0.00237

MarkerSeek score 61.30

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1285
variable_sites: 3
pi: 0.00237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCCGTCAGTAGAAAACCG	50.0	50.0	57.9	58.2	1421-1432 bp	1.0	79.3
psbE-petL_p2	CAACCCGCAATGAATAGGGA	GCCGTCAGTAGAAAACCGAA	50.0	50.0	57.9	58.2	1419-1430 bp	1.0	79.2
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	AAGCCGTCAGTAGAAAACCG	50.0	50.0	57.8	58.2	1344-1355 bp	1.0	78.5
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GCCGTCAGTAGAAAACCGAA	50.0	50.0	57.8	58.2	1342-1353 bp	1.0	78.5
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AAGCCGTCAGTAGAAAACCG	45.0	50.0	58.2	58.2	1430-1441 bp	1.0	78.3