Hotspot

ndhC-trnV-UAC

LSC · 67805-68969 · 1165 bp

Back to Cichorium

Pi 0.00431

MarkerSeek score 61.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1165
variable_sites: 5
pi: 0.00431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGCTAATCGGAGCT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1313-1360 bp	1.0	77.2
ndhC-trnV-UAC_p2	CGGTCCTTTGCTAATCGGAG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.4	58.1	1315-1362 bp	1.0	76.3
ndhC-trnV-UAC_p3	CTCCGGTCCTTTGCTAATCG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.4	58.1	1318-1365 bp	1.0	76.3
ndhC-trnV-UAC_p4	GGTCCTTTGCTAATCGGAGC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.7	58.1	1314-1361 bp	1.0	74.1
ndhC-trnV-UAC_p5	GTCCTTTGCTAATCGGAGCT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.7	58.7	1298-1345 bp	1.0	72.6