primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	trnK-UUU	1	ATATGCCCGTGTCAACCAAT	TGGGTTCGTTGGATTAGCTT	20	20	45.0	45.0	57.6	57.1	0.0	0.0	1.306469	19363	21957	2971-2979	1.000000	70.0
trnK-UUU_p2	trnK-UUU	2	ATATGCCCGTGTCAACCAAT	GATGGGTTCGTTGGATTAGCT	20	21	45.0	47.6	57.6	58.1	0.0	0.0	1.516145	19363	21957	2973-2981	1.000000	68.3
trnK-UUU_p3	trnK-UUU	3	ATATGCCCGTGTCAACCAAT	ATGGGTTCGTTGGATTAGCT	20	20	45.0	45.0	57.6	56.9	0.0	0.0	1.537214	19363	21957	2972-2980	1.000000	68.1
trnK-UUU_p4	trnK-UUU	4	ATATGCCCGTGTCAACCAAT	TCAAATGATGGGTTCGTTGGA	20	21	45.0	42.9	57.6	57.9	0.0	0.0	1.582926	19363	21957	2979-2987	1.000000	67.7
trnK-UUU_p5	trnK-UUU	5	ATGCCCGTGTCAACCAATAC	TGGGTTCGTTGGATTAGCTT	20	20	50.0	45.0	58.5	57.1	0.0	0.0	1.411646	19363	21957	2969-2969	0.500000	48.7
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CCGATGGTATGGACGAATCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	21958	22750	996-1001	1.000000	77.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTATGGACGAATCCC	TTCCTTGAAAAAGGCGCTCA	20	20	55.0	45.0	57.9	58.3	0.0	7.6	0.393931	21958	22750	995-1000	1.000000	77.8
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	21958	22750	936-941	1.000000	77.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	58.2	58.3	20.3	7.6	0.482065	21958	22750	935-940	1.000000	77.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	20	20	50.0	45.0	57.8	58.3	0.0	7.6	0.547945	21958	22750	1020-1025	1.000000	76.6
rps16_p1	rps16	1	AGAGCAAGAAAAGAAGGAAA	TCTCTGTACCGACTGAACCA	20	20	35.0	50.0	52.6	57.7	0.0	0.0	5.691513	22751	23878	1297-1310	1.000000	40.6
rps16_p2	rps16	2	AGAGCAAGAAAAGAAGGAAAT	TCTCTGTACCGACTGAACCA	21	20	33.3	50.0	53.3	57.7	0.0	0.0	5.984059	22751	23878	1297-1310	1.000000	40.6
rps16_p3	rps16	3	AGAGCAAGAAAAGAAGGAAA	GTCTCTGTACCGACTGAACC	20	20	35.0	55.0	52.6	57.4	0.0	0.0	6.043173	22751	23878	1298-1311	1.000000	40.6
rps16_p4	rps16	4	AGAAATCACTATCCTACCCTTAGT	TCTCTGTACCGACTGAACCA	24	20	37.5	50.0	56.1	57.7	0.0	0.0	6.184942	22751	23878	1238-1251	1.000000	40.6
rps16_p5	rps16	5	AGAAATCACTATCCTACCCT	TCTCTGTACCGACTGAACCA	20	20	40.0	50.0	52.0	57.7	0.0	0.0	6.245418	22751	23878	1238-1251	1.000000	40.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	23879	24855	1050-1050	1.000000	78.6
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	7.8	16.9	0.445953	23879	24855	1065-1065	1.000000	76.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	20	20	55.0	55.0	58.4	58.1	2.9	16.9	0.445953	23879	24855	1064-1064	1.000000	76.7
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	23879	24855	1054-1054	1.000000	73.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	23879	24855	1080-1080	1.000000	73.7
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	26231	27008	907-929	1.000000	80.2
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	26231	27008	889-911	1.000000	78.1
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.718384	26231	27008	843-865	1.000000	76.5
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.756923	26231	27008	908-930	1.000000	76.0
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.6	58.4	0.0	14.6	0.991747	26231	27008	825-847	1.000000	74.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	27081	27914	891-893	1.000000	80.5
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	27081	27914	909-911	1.000000	78.2
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	27081	27914	890-892	1.000000	74.5
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	27081	27914	899-901	1.000000	74.4
trnC-GCA-petN_p5	trnC-GCA-petN	5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.063167	27081	27914	948-950	1.000000	73.7
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.6	58.3	0.0	0.0	3.700923	28595	29261	780-786	1.000000	51.3
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	20	20	40.0	50.0	54.5	58.3	0.0	0.0	3.756773	28595	29261	774-780	1.000000	50.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.4	58.3	0.0	0.0	3.875520	28595	29261	777-783	1.000000	49.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGGAACTAAAATGAACAGTGCAG	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.3	58.3	0.0	0.0	3.958013	28595	29261	781-787	1.000000	49.3
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGGAACTAAAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	22	20	36.4	50.0	56.2	58.3	0.0	0.0	4.069095	28595	29261	781-787	1.000000	48.4
trnE-UUC-rpoB_p1	trnE-UUC-rpoB	1	GGGACTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.0	56.8	0.0	0.0	2.237066	29709	30560	993-995	1.000000	62.7
trnE-UUC-rpoB_p2	trnE-UUC-rpoB	2	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCTGA	20	21	55.0	42.9	58.1	56.8	14.8	0.0	2.326325	29709	30560	952-954	1.000000	62.1
trnE-UUC-rpoB_p3	trnE-UUC-rpoB	3	CCCTCGTTTTCTCCGTGAAA	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	29709	30560	983-985	1.000000	61.9
trnE-UUC-rpoB_p4	trnE-UUC-rpoB	4	TTGAACCCTCGTTTTCTCCG	AGTTCTTCTGTCAAACCCTGA	20	21	50.0	42.9	58.1	56.8	0.0	0.0	2.340425	29709	30560	988-990	1.000000	61.9
trnE-UUC-rpoB_p5	trnE-UUC-rpoB	5	GGGACTTGAACCCTCGTTTT	TCAAACCCTGATCAATGAACCT	20	22	50.0	40.9	58.0	57.6	0.0	0.0	2.409970	29709	30560	983-985	1.000000	61.4
rpoC1_p1	rpoC1	1	ACGATCTTTAGCTCTGGAACTG	ACAATGGGCTTGAGTCAGTG	22	20	45.5	50.0	57.9	58.1	0.0	0.0	2.215156	33770	36575	2989-2990	1.000000	62.5
rpoC1_p2	rpoC1	2	TCTGAGAAGACTTTCCAGCT	ACAATGGGCTTGAGTCAGTG	20	20	45.0	50.0	55.8	58.1	11.0	0.0	2.302486	33770	36575	2952-2953	1.000000	61.8
rpoC1_p3	rpoC1	3	ACGATCTTTAGCTCTGGAACTG	CAATGGGCTTGAGTCAGTGA	22	20	45.5	50.0	57.9	57.8	0.0	0.0	2.306340	33770	36575	2988-2989	1.000000	61.7
rpoC1_p4	rpoC1	4	ACGATCTTTAGCTCTGGAACTG	TGGGCTTGAGTCAGTGATTG	22	20	45.5	50.0	57.9	57.8	0.0	0.0	2.306340	33770	36575	2985-2986	1.000000	61.7
rpoC1_p5	rpoC1	5	GTTCGTTCGAGAACTACGATCT	TGGGCTTGAGTCAGTGATTG	22	20	45.5	50.0	58.0	57.8	9.8	0.0	2.190611	33770	36575	3000-3000	0.500000	42.5
atpI-atpH_p1	atpI-atpH	1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	20	20	50.0	45.0	58.3	58.0	0.0	1.4	0.297359	42745	43884	1270-1276	1.000000	78.8
atpI-atpH_p2	atpI-atpH	2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.371251	42745	43884	1326-1332	1.000000	78.2
atpI-atpH_p3	atpI-atpH	3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.407418	42745	43884	1286-1292	1.000000	77.9
atpI-atpH_p4	atpI-atpH	4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	20	20	50.0	45.0	58.3	58.2	0.0	1.4	0.442898	42745	43884	1271-1277	1.000000	77.6
atpI-atpH_p5	atpI-atpH	5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.449288	42745	43884	1330-1336	1.000000	77.5
trnT-TGT-trnL-UAA_p1	trnT-TGT-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	63979	64539	622-629	1.000000	78.2
trnT-TGT-trnL-UAA_p2	trnT-TGT-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	63979	64539	654-661	1.000000	77.3
trnT-TGT-trnL-UAA_p3	trnT-TGT-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	63979	64539	616-623	1.000000	76.9
trnT-TGT-trnL-UAA_p4	trnT-TGT-trnL-UAA	4	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	15.5	0.0	0.651279	63979	64539	648-655	1.000000	76.0
trnT-TGT-trnL-UAA_p5	trnT-TGT-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	CGTAGCGTCTACCGATTTCG	20	20	55.0	55.0	57.9	58.6	0.0	10.2	0.749620	63979	64539	621-628	1.000000	75.3
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	65504	66128	728-728	1.000000	70.1
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	65504	66128	727-727	1.000000	70.1
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	20	21	50.0	47.6	58.1	57.7	0.0	0.0	1.349030	65504	66128	735-735	1.000000	69.5
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	20	21	50.0	47.6	58.1	57.5	0.0	3.3	1.537359	65504	66128	729-729	1.000000	68.0
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	20	21	50.0	47.6	58.1	58.6	0.0	3.3	1.647337	65504	66128	728-728	1.000000	67.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GTCCTTTGCTAATCGGAGCT	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.7	58.1	0.0	0.0	0.425062	67805	68969	1313-1360	1.000000	77.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CGGTCCTTTGCTAATCGGAG	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.4	58.1	0.0	0.0	0.535614	67805	68969	1315-1362	1.000000	76.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTCCGGTCCTTTGCTAATCG	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.4	58.1	0.0	0.0	0.535614	67805	68969	1318-1365	1.000000	76.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	GGTCCTTTGCTAATCGGAGC	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.7	58.1	3.4	0.0	0.807051	67805	68969	1314-1361	1.000000	74.1
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GTCCTTTGCTAATCGGAGCT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.7	58.7	0.0	0.0	1.000879	67805	68969	1298-1345	1.000000	72.6
atpB-rbcL_p1	atpB-rbcL	1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.047103	71976	72733	918-919	1.000000	72.0
atpB-rbcL_p2	atpB-rbcL	2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.0	58.1	0.0	0.0	1.080391	71976	72733	917-918	1.000000	71.7
atpB-rbcL_p3	atpB-rbcL	3	CGCCCCAGTTTCTTTTTGTC	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.151466	71976	72733	871-872	1.000000	71.2
atpB-rbcL_p4	atpB-rbcL	4	CGCCCCAGTTTCTTTTTGTC	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	57.9	58.1	0.0	0.0	1.184754	71976	72733	870-871	1.000000	70.9
atpB-rbcL_p5	atpB-rbcL	5	TTCTTTTTGTCAAGCGTGGC	CACCAGCTTTGAATCCAACAC	20	21	45.0	47.6	57.8	58.0	0.0	0.0	1.219560	71976	72733	862-863	1.000000	70.6
accD_p1	accD	1	TCATGCAAATATGGAGCAAGA	ACTTGCTTGCTCAAAATAATTGA	21	23	38.1	30.4	55.6	55.6	0.0	0.0	8.720303	74703	76109	1475-1487	1.000000	41.2
accD_p2	accD	2	TCATGCAAATATGGAGCAAGA	TGCTTGCTCAAAATAATTGAACT	21	23	38.1	30.4	55.6	55.6	0.0	0.0	8.720303	74703	76109	1472-1484	1.000000	41.2
accD_p3	accD	3	TCATGCAAATATGGAGCAAGA	ACTTGCTTGCTCAAAATAATTGAAC	21	25	38.1	32.0	55.6	57.6	0.0	0.0	8.781346	74703	76109	1475-1487	1.000000	41.2
accD_p4	accD	4	TCATGCAAATATGGAGCAAGAA	ACTTGCTTGCTCAAAATAATTGA	22	23	36.4	30.4	56.4	55.6	0.0	0.0	8.962004	74703	76109	1475-1487	1.000000	41.2
accD_p5	accD	5	TCATGCAAATATGGAGCAAGAA	TGCTTGCTCAAAATAATTGAACT	22	23	36.4	30.4	56.4	55.6	0.0	0.0	8.962004	74703	76109	1472-1484	1.000000	41.2
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCCGTCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.2	6.4	0.0	0.259329	82182	83466	1421-1432	1.000000	79.3
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCCGTCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.2	6.4	0.0	0.264322	82182	83466	1419-1430	1.000000	79.2
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	AAGCCGTCAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.2	24.9	0.0	0.364337	82182	83466	1344-1355	1.000000	78.5
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	GCCGTCAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.2	24.9	0.0	0.369331	82182	83466	1342-1353	1.000000	78.5
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	AAGCCGTCAGTAGAAAACCG	20	20	45.0	50.0	58.2	58.2	0.0	0.0	0.379015	82182	83466	1430-1441	1.000000	78.3