Hotspot

ycf15-rps12

IRa · 137651-138668 · 1018 bp

Back to Cicerbita

Pi 0.00378
MarkerSeek score 64.60
Primer available yes
Label ycf15-rps12

Feature Metadata

label_name
ycf15-rps12
feature_type
igs
region
IRa
length_bp
1018
variable_sites
6
pi
0.00378
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-rps12_p1 GAAAGGACTCACTAAGCCGG TGGCAACTTTACGTAAGGCA 55.0 45.0 58.0 57.7 1057-1094 bp 1.0 81.0
ycf15-rps12_p2 CACGGTACAATCCTCTTCCC TGGCAACTTTACGTAAGGCA 55.0 45.0 57.7 57.7 1146-1183 bp 1.0 78.3
ycf15-rps12_p3 GAAAGGACTCACTAAGCCGG ACGTAAGGCAGAGTTTGGTT 55.0 45.0 58.0 57.4 1047-1084 bp 1.0 78.0
ycf15-rps12_p4 TCACTAAGCCGGGATCACTA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.6 57.7 1049-1086 bp 1.0 77.5
ycf15-rps12_p5 CCCTTTCTCCTCTGTCCACA TGGCAACTTTACGTAAGGCA 55.0 45.0 58.6 57.7 1118-1155 bp 1.0 75.7