Hotspot

trnC-GCA-petN

LSC · 9431-10246 · 816 bp

Back to Cicerbita

Pi 0.0167
MarkerSeek score 62.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
816
variable_sites
25
pi
0.0167
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 872-877 bp 1.0 81.0
trnC-GCA-petN_p2 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 890-895 bp 1.0 78.7
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG GCCCAAGCGAGACTTACTAT 50.0 50.0 58.1 57.1 871-876 bp 1.0 75.0
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG ATTAAAGCAGCCCAAGCGAG 50.0 50.0 58.1 58.9 880-885 bp 1.0 74.9
trnC-GCA-petN_p5 ATTGTGTATCGTTTTGGCGG AGCCCAAGCGAGACTTACTA 45.0 50.0 57.1 58.2 929-934 bp 1.0 74.2