Hotspot

trnK-UUU-rps16

LSC · 4344-5164 · 821 bp

Back to Cicerbita

Pi 0.016
MarkerSeek score 64.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
821
variable_sites
23
pi
0.016
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGAACTTTGCCCTAATCAAACG 50.0 45.5 58.2 58.2 876-906 bp 1.0 66.8
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGAACTTTGCCCTAATCAAACG 50.0 45.5 58.2 58.2 875-905 bp 1.0 66.8
trnK-UUU-rps16_p3 TCCTAGCCGCACTTAAAAGC GGAACTTTGCCCTAATCAAACG 50.0 45.5 58.3 58.2 891-921 bp 1.0 65.9
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA GGAACTTTGCCCTAATCAAACG 50.0 45.5 58.3 58.2 885-915 bp 1.0 65.8
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GGAACTTTGCCCTAATCAAACG 50.0 45.5 57.5 58.2 862-892 bp 1.0 64.5