primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GGAACTTTGCCCTAATCAAACG	20	22	50.0	45.5	58.2	58.2	20.3	0.0	2.334977	4344	5164	876-906	1.000000	66.8
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GGAACTTTGCCCTAATCAAACG	20	22	50.0	45.5	58.2	58.2	20.3	0.0	2.334977	4344	5164	875-905	1.000000	66.8
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCCTAGCCGCACTTAAAAGC	GGAACTTTGCCCTAATCAAACG	20	22	50.0	45.5	58.3	58.2	0.0	0.0	2.432395	4344	5164	891-921	1.000000	65.9
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	GGAACTTTGCCCTAATCAAACG	20	22	50.0	45.5	58.3	58.2	5.3	0.0	2.449364	4344	5164	885-915	1.000000	65.8
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TACCGTTGAGTTAGCAACCC	GGAACTTTGCCCTAATCAAACG	20	22	50.0	45.5	57.5	58.2	0.0	0.0	2.627315	4344	5164	862-892	1.000000	64.5
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.233476	8610	9359	879-906	1.000000	83.8
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.2	58.4	0.0	14.6	0.506839	8610	9359	861-888	1.000000	81.8
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	20	20	55.0	50.0	58.6	58.1	0.0	0.0	0.718384	8610	9359	815-842	1.000000	80.4
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	57.3	58.1	0.0	0.0	0.756923	8610	9359	880-907	1.000000	79.6
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	20	20	55.0	50.0	58.6	58.4	0.0	14.6	0.991747	8610	9359	797-824	1.000000	78.4
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9431	10246	872-877	1.000000	81.0
trnC-GCA-petN_p2	trnC-GCA-petN	2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	9431	10246	890-895	1.000000	78.7
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9431	10246	871-876	1.000000	75.0
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	20	20	50.0	50.0	58.1	58.9	0.0	0.0	0.984078	9431	10246	880-885	1.000000	74.9
trnC-GCA-petN_p5	trnC-GCA-petN	5	ATTGTGTATCGTTTTGGCGG	AGCCCAAGCGAGACTTACTA	20	20	45.0	50.0	57.1	58.2	0.0	0.0	1.063167	9431	10246	929-934	1.000000	74.2
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.2	55.6	0.0	0.0	2.573941	10337	10802	628-642	1.000000	62.0
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTTT	20	21	50.0	38.1	58.2	56.4	0.0	0.0	2.785752	10337	10802	628-642	1.000000	60.4
petN-psbM_p3	petN-psbM	3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	57.1	55.6	0.0	0.0	3.315377	10337	10802	629-643	1.000000	56.1
petN-psbM_p4	petN-psbM	4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTT	20	20	50.0	40.0	58.9	55.6	0.0	0.0	3.324543	10337	10802	620-634	1.000000	56.1
petN-psbM_p5	petN-psbM	5	TGGGCTGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTT	21	20	47.6	40.0	58.0	55.6	0.0	0.0	3.423261	10337	10802	614-628	1.000000	55.3
atpI-atpH_p1	atpI-atpH	1	TCCCTGTCATGTTCCTTGGA	ATAACGGAAGCGGCAGAAAT	20	20	50.0	45.0	58.3	58.0	0.0	1.4	0.297359	25139	26271	1269-1283	1.000000	79.5
atpI-atpH_p2	atpI-atpH	2	TCCCTGTCATGTTCCTTGGA	TACCTTGACCAACTCCAGGT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.371251	25139	26271	1325-1339	1.000000	78.8
atpI-atpH_p3	atpI-atpH	3	TCCCTGTCATGTTCCTTGGA	AGCCAATCCAGCAGCAATAA	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.407418	25139	26271	1285-1299	1.000000	78.6
atpI-atpH_p4	atpI-atpH	4	TCCCTGTCATGTTCCTTGGA	AATAACGGAAGCGGCAGAAA	20	20	50.0	45.0	58.3	58.2	0.0	1.4	0.442898	25139	26271	1270-1284	1.000000	78.3
atpI-atpH_p5	atpI-atpH	5	TCCCTGTCATGTTCCTTGGA	GCAGTACCTTGACCAACTCC	20	20	50.0	55.0	58.3	58.2	0.0	0.0	0.449288	25139	26271	1329-1343	1.000000	78.2
trnG-UCC-trnT_p1	trnG-UCC-trnT	1	AATCAAACCGAAAGACCCCT	ATTTGAACCGAGGGCTTACG	20	20	45.0	50.0	57.0	58.3	0.0	0.0	1.237738	30936	31113	304-328	1.000000	76.4
trnG-UCC-trnT_p2	trnG-UCC-trnT	2	ATCAAACCGAAAGACCCCTT	ATTTGAACCGAGGGCTTACG	20	20	45.0	50.0	57.0	58.3	0.0	0.0	1.237738	30936	31113	303-327	1.000000	76.4
trnG-UCC-trnT_p3	trnG-UCC-trnT	3	AATCAAACCGAAAGACCCCT	GGATTTGAACCGAGGGCTTA	20	20	45.0	50.0	57.0	57.6	0.0	0.0	1.385210	30936	31113	306-330	1.000000	75.2
trnG-UCC-trnT_p4	trnG-UCC-trnT	4	ATCAAACCGAAAGACCCCTT	GGATTTGAACCGAGGGCTTA	20	20	45.0	50.0	57.0	57.6	0.0	0.0	1.385210	30936	31113	305-329	1.000000	75.2
trnG-UCC-trnT_p5	trnG-UCC-trnT	5	GAATCAAACCGAAAGACCCCT	ATTTGAACCGAGGGCTTACG	21	20	47.6	50.0	58.2	58.3	0.0	0.0	1.474182	30936	31113	305-329	1.000000	74.5
psaA-ycf3_p1	psaA-ycf3	1	GAGAAATGACCCGGTCTAGC	AGCCGCTGAGTATTGGAAAC	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.487535	41398	42130	924-926	1.000000	77.0
psaA-ycf3_p2	psaA-ycf3	2	GAGAAATGACCCGGTCTAGC	CAAGCCGCTGAGTATTGGAA	20	20	55.0	50.0	57.8	58.3	18.5	0.0	0.488764	41398	42130	926-928	1.000000	77.0
psaA-ycf3_p3	psaA-ycf3	3	GAGAAATGACCCGGTCTAGC	AAGCCGCTGAGTATTGGAAA	20	20	55.0	45.0	57.8	57.5	18.5	0.0	0.716540	41398	42130	925-927	1.000000	75.1
psaA-ycf3_p4	psaA-ycf3	4	TTTTACTTCTGGTTCCGGCG	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.745852	41398	42130	859-861	1.000000	75.0
psaA-ycf3_p5	psaA-ycf3	5	TTTTACTTCTGGTTCCGGCG	CAAGCCGCTGAGTATTGGAA	20	20	50.0	50.0	58.5	58.3	0.0	0.0	0.747080	41398	42130	861-863	1.000000	75.0
trnF-ndhJ_p1	trnF-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.273485	47924	48560	663-766	1.000000	75.4
trnF-ndhJ_p2	trnF-ndhJ	2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	20	20	50.0	50.0	58.1	56.8	0.0	3.3	1.275704	47924	48560	662-765	1.000000	75.3
trnF-ndhJ_p3	trnF-ndhJ	3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	20	21	50.0	47.6	58.1	57.7	0.0	0.0	1.349030	47924	48560	670-773	1.000000	74.7
trnF-ndhJ_p4	trnF-ndhJ	4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	20	21	50.0	47.6	58.1	57.5	0.0	3.3	1.537359	47924	48560	664-767	1.000000	73.2
trnF-ndhJ_p5	trnF-ndhJ	5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	20	21	50.0	47.6	58.1	58.6	0.0	3.3	1.647337	47924	48560	663-766	1.000000	72.4
ycf4-cemA_p1	ycf4-cemA	1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.106483	60255	61039	964-973	1.000000	81.0
ycf4-cemA_p2	ycf4-cemA	2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	58.1	58.1	3.3	0.0	0.172357	60255	61039	896-905	1.000000	80.6
ycf4-cemA_p3	ycf4-cemA	3	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.172357	60255	61039	928-937	1.000000	80.5
ycf4-cemA_p4	ycf4-cemA	4	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.251008	60255	61039	910-919	1.000000	79.9
ycf4-cemA_p5	ycf4-cemA	5	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	20	20	45.0	50.0	57.8	58.1	1.4	0.0	0.297297	60255	61039	908-917	1.000000	79.6
rpl33-rps18_p1	rpl33-rps18	1	CGATTCACGGGGAGGTAAAG	TCAATTCGATCCCCCGATTG	20	20	55.0	50.0	58.1	57.7	0.0	0.0	0.318613	67633	67816	289-296	1.000000	80.2
rpl33-rps18_p2	rpl33-rps18	2	GGCACAATACGCCTAATCGA	TCAATTCGATCCCCCGATTG	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.393870	67633	67816	349-356	1.000000	79.1
rpl33-rps18_p3	rpl33-rps18	3	ACTCAAAAGAACCGGCACAA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	58.2	57.7	0.0	0.0	0.496864	67633	67816	362-369	1.000000	78.2
rpl33-rps18_p4	rpl33-rps18	4	CGATTCACGGGGAGGTAAAG	ATTCGATCCCCCGATTGGAT	20	20	55.0	50.0	58.1	58.6	0.0	0.0	0.701843	67633	67816	286-293	1.000000	77.2
rpl33-rps18_p5	rpl33-rps18	5	GGCACAATACGCCTAATCGA	ATTCGATCCCCCGATTGGAT	20	20	50.0	50.0	58.1	58.6	0.0	0.0	0.777100	67633	67816	346-353	1.000000	76.1
rps3_p1	rps3	1	ATTGGTTTCTGGTCGGTTCC	AAGCGCGAAGGGTAATTGAT	20	20	50.0	45.0	58.1	58.0	0.0	12.1	0.132749	82569	83225	1786-1797	1.000000	80.6
rps3_p2	rps3	2	ATTGGTTTCTGGTCGGTTCC	TCCCGGGCATCTACCATTAT	20	20	50.0	50.0	58.1	58.0	0.0	18.5	0.133225	82569	83225	2091-2102	1.000000	80.5
rps3_p3	rps3	3	ATTGGTTTCTGGTCGGTTCC	GAGGGCGTAGTTTTGCGATA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.163795	82569	83225	1564-1575	1.000000	80.5
rps3_p4	rps3	4	TTGTTTATACCGTGGGGCTG	AAGCGCGAAGGGTAATTGAT	20	20	50.0	45.0	57.9	58.0	0.0	12.1	0.155214	82569	83225	1271-1271	1.000000	79.8
rps3_p5	rps3	5	TTGTTTATACCGTGGGGCTG	TCCCGGGCATCTACCATTAT	20	20	50.0	50.0	57.9	58.0	0.0	18.5	0.155690	82569	83225	1576-1578	1.000000	79.8
rps12-ycf15_p1	rps12-ycf15	1	TGGCAACTTTACGTAAGGCA	GAAAGGACTCACTAAGCCGG	20	20	45.0	55.0	57.7	58.0	16.5	0.0	0.269231	97919	98936	1057-1094	1.000000	81.0
rps12-ycf15_p2	rps12-ycf15	2	TGGCAACTTTACGTAAGGCA	CACGGTACAATCCTCTTCCC	20	20	45.0	55.0	57.7	57.7	16.5	0.0	0.568815	97919	98936	1146-1183	1.000000	78.3
rps12-ycf15_p3	rps12-ycf15	3	ACGTAAGGCAGAGTTTGGTT	GAAAGGACTCACTAAGCCGG	20	20	45.0	55.0	57.4	58.0	0.0	0.0	0.644527	97919	98936	1047-1084	1.000000	78.0
rps12-ycf15_p4	rps12-ycf15	4	TGGCAACTTTACGTAAGGCA	TCACTAAGCCGGGATCACTA	20	20	45.0	50.0	57.7	57.6	16.5	2.2	0.705099	97919	98936	1049-1086	1.000000	77.5
rps12-ycf15_p5	rps12-ycf15	5	TGGCAACTTTACGTAAGGCA	CCCTTTCTCCTCTGTCCACA	20	20	45.0	55.0	57.7	58.6	16.5	0.0	0.905608	97919	98936	1118-1155	1.000000	75.7
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGTCGGAAAAAGAAG	CCGCCCAATATCCTTTCCTT	20	20	50.0	50.0	58.0	57.6	17.5	0.0	0.397386	111300	112356	1233-1243	1.000000	79.5
ndhF-rpl32_p2	ndhF-rpl32	2	TGCCGTCGGAAAAAGAAGAA	CCGCCCAATATCCTTTCCTT	20	20	45.0	50.0	58.0	57.6	17.5	0.0	0.408676	111300	112356	1231-1241	1.000000	79.5
ndhF-rpl32_p3	ndhF-rpl32	3	TTGCCGTCGGAAAAAGAAGA	CCGCCCAATATCCTTTCCTT	20	20	45.0	50.0	58.0	57.6	17.5	0.0	0.408676	111300	112356	1232-1242	1.000000	79.4
ndhF-rpl32_p4	ndhF-rpl32	4	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	20	20	50.0	45.0	58.0	57.6	17.5	9.5	0.425347	111300	112356	1250-1260	1.000000	79.3
ndhF-rpl32_p5	ndhF-rpl32	5	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	20	20	45.0	45.0	58.0	57.6	17.5	9.5	0.436636	111300	112356	1248-1258	1.000000	79.2
rpl32-trnL_p1	rpl32-trnL	1	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.372803	112522	113413	1022-1062	1.000000	82.0
rpl32-trnL_p2	rpl32-trnL	2	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.374173	112522	113413	1023-1063	1.000000	81.9
rpl32-trnL_p3	rpl32-trnL	3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.400763	112522	113413	1005-1045	1.000000	81.8
rpl32-trnL_p4	rpl32-trnL	4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	20	20	45.0	50.0	57.6	58.0	13.5	0.0	0.402133	112522	113413	1006-1046	1.000000	81.8
rpl32-trnL_p5	rpl32-trnL	5	AAGGAAAGGATATTGGGCGG	TTAGAAGATGTCATGCCGCC	20	20	50.0	50.0	57.6	58.0	0.0	0.0	0.411413	112522	113413	1086-1126	1.000000	81.4
ycf1_p1	ycf1	1	TCGAGGAGGACCAAAAGGAT	GAAATCCTCATTCCCCGCTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.105971	125833	125952	584-584	1.000000	83.7
ycf1_p2	ycf1	2	CGAGGAGGACCAAAAGGATG	GAAATCCTCATTCCCCGCTT	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.088431	125833	125952	583-583	0.500000	59.6
ycf1_p3	ycf1	3	CGAGGAGGACCAAAAGGATG	ACCAGATTTTCGTCGATCCG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.107268	125833	125952	663-663	0.500000	59.4
ycf1_p4	ycf1	4	CGAGGAGGACCAAAAGGATG	CCAGATTTTCGTCGATCCGT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.107268	125833	125952	662-662	0.500000	59.4
ycf1_p5	ycf1	5	TCGAGGAGGACCAAAAGGAT	CCAGATTTTCGTCGATCCGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.124808	125833	125952	663-663	0.500000	59.2
ycf15-rps12_p1	ycf15-rps12	1	GAAAGGACTCACTAAGCCGG	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.0	57.7	0.0	16.5	0.269231	137651	138668	1057-1094	1.000000	81.0
ycf15-rps12_p2	ycf15-rps12	2	CACGGTACAATCCTCTTCCC	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	57.7	57.7	0.0	16.5	0.568815	137651	138668	1146-1183	1.000000	78.3
ycf15-rps12_p3	ycf15-rps12	3	GAAAGGACTCACTAAGCCGG	ACGTAAGGCAGAGTTTGGTT	20	20	55.0	45.0	58.0	57.4	0.0	0.0	0.644527	137651	138668	1047-1084	1.000000	78.0
ycf15-rps12_p4	ycf15-rps12	4	TCACTAAGCCGGGATCACTA	TGGCAACTTTACGTAAGGCA	20	20	50.0	45.0	57.6	57.7	2.2	16.5	0.705099	137651	138668	1049-1086	1.000000	77.5
ycf15-rps12_p5	ycf15-rps12	5	CCCTTTCTCCTCTGTCCACA	TGGCAACTTTACGTAAGGCA	20	20	55.0	45.0	58.6	57.7	0.0	16.5	0.905608	137651	138668	1118-1155	1.000000	75.7