Hotspot

ycf2

Genome · 39974-45877 · 5904 bp

Pi 0.0355

MarkerSeek score 65.70

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 5904
variable_sites: 347
pi: 0.0355
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CATGGTTCGATCCTGCAAGA	CCGACAGAAAGTCCGTTTGA	50.0	50.0	58.0	58.1	363-375 bp	0.75	79.2
ycf2_p2	CATGGTTCGATCCTGCAAGA	TTTTGAGTTGTTCTGCCGGA	50.0	45.0	58.0	58.0	518-530 bp	0.75	78.7
ycf2_p3	GGTTCGATCCTGCAAGAACT	TTTTGAGTTGTTCTGCCGGA	50.0	45.0	57.9	58.0	515-527 bp	0.75	78.2
ycf2_p4	GGCTTATTCGAAAAACGCCC	TTTTGAGTTGTTCTGCCGGA	50.0	45.0	58.1	58.0	573-585 bp	0.75	77.8
ycf2_p5	CCCGAAATGACCTTTCCCAA	TTTTGAGTTGTTCTGCCGGA	50.0	45.0	58.1	58.0	456 bp	0.5	61.8